antibody information anti rabbit aromatase  (Novus Biologicals)

Journal: Frontiers in Veterinary Science

Article Title: MNQ derivative D19 alleviates LPS-induced inflammation and oxidative stress in sheep follicular granulosa cells through the GPX4 -mediated ferroptosis

doi: 10.3389/fvets.2025.1621738

Figure Lengend Snippet: D19 restoring LPS-suppressed steroid hormone production in GCs. (A) ELISA measurement of A 4 , E 2 , and P 4 levels in GCs. (B,C) qRT-PCR and Western blotting were employed to detect the relative expression levels of mRNA and protein, respectively, for genes involved in steroid hormone synthesis ( HSD17B4 , CYP19A1 , 3β-HSD , CYP11A1 , and STAR ). Data from at least three independent experiments are presented as mean ± SEM. Significant differences ( p < 0.05) are denoted by different letters.

Article Snippet: CYP19A1 , bs-0114R , Bioss , Rabbit , 1:500.

Techniques: Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot, Expressing

Journal: Frontiers in Veterinary Science

Article Title: MNQ derivative D19 alleviates LPS-induced inflammation and oxidative stress in sheep follicular granulosa cells through the GPX4 -mediated ferroptosis

doi: 10.3389/fvets.2025.1621738

Figure Lengend Snippet: D19 preventing GPX4 deficiency-induced disruption of steroidogenesis in GCs. (A) Levels of E 2 and P 4 in different treatment groups were measured using ELISA. (B) The relative mRNA and protein expression levels of steroid hormone synthesis-related genes ( HSD17B4 , CYP19A1 , 3β-HSD , CYP11A1 , and STAR ) were detected by qRT-PCR and Western blotting, respectively. All experiments were performed in triplicate, and data are presented as mean ± SEM. Different letters indicate statistically significant differences ( p < 0.05).

Article Snippet: CYP19A1 , bs-0114R , Bioss , Rabbit , 1:500.

Techniques: Disruption, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative RT-PCR, Western Blot

Journal: Molecular cancer therapeutics

Article Title: Preclinical Evaluation of the Efficacy of a Cyclin-dependent Kinase Inhibitor Ribociclib in Combination with Letrozole Against Patient-Derived Glioblastoma Cells

doi: 10.1158/1535-7163.MCT-25-0277

Figure Lengend Snippet: A, Oncoprint of CDKN2A, CDKN2B, CDK4, CDK6 and RB1 gene expression in GBM samples from the TCGA, Cell 2013 dataset (577), accessed via cBioPortal. This Oncoprint displays alterations (amplifications, deletions, and mutations) in these key cell cycle regulators across GBM samples, highlighting the frequency and distribution of expression changes within the CDK4/6 pathway in GBM. The methylation status of the samples from the selected dataset and the comparison of the overall survival between the CDKN2A, CDK4 (altered) vs the unaltered group. B, RNA-seq analysis of FFPE tumor samples from our Phase 0/1 clinical trial of LTZ in recurrent GBM patients ( NCT03122197 ). Differential gene expression between the 2.5mg (d2p5) vs the 5 (d5),10 (d10),12.5 9d12.5p5) and 15mg (d15) LTZ-treated tumors. Genes upregulated after LTZ treatment, associated with cell cycle and DNA repair. C, Log2 Counts Per Million (log2CPM) represents normalized expression level of CDK4 and CDKN2A in each sample across the entire dose cohort D, Log2 Fold Change (log2FC) represents relative change in CDK4 and CDKN2A expression between the 2.5mg vs the 5,10,12.5 and 15mg LTZ treated cohort. E, Western blot analysis of Aromatase expression in the patient-derived GBM lines used in the study vs MCF7 (ER +) and MDA-MB-468 (ER +) cell lines.

Article Snippet: Proteins were separated by SDS-PAGE, transferred using the Trans-Blot Turbo system (Bio-Rad), and probed with anti-aromatase antibody (1:250, Cell Signaling, Cat# 14528) followed by IRDye ® 800CW Goat anti-Rabbit secondary (1:1000, LI-COR ® , Cat# 926–32211) in 5% BSA.

Techniques: Gene Expression, Expressing, Methylation, Comparison, RNA Sequencing, Western Blot, Derivative Assay

Journal: Animals : an Open Access Journal from MDPI

Article Title: Apricot Bee Pollen Alleviates Deoxynivalenol-Induced Cellular Toxicity in Bovine Granulosa Cells

doi: 10.3390/ani15111580

Figure Lengend Snippet: The toxic effects of DON on steroid hormones and oxidation reduction in bGCs. ( A , B ) Cells were treated with various concentrations of DON (0, 0.1, 0.5, and 1 μmol/L) for 24 h, after which the levels of estradiol and progesterone in the supernatant were measured by ELISA. Data were normalized based on the protein concentration of each sample well and expressed as fold changes relative to the control. ( C ) Cells were treated with 0.5 μmol/L DON for 24 h and Western blot analysis was conducted. The quantitative results were presented as fold changes in the ratios of CYP19A1/GAPDH, CYP11A1/GAPDH, and StAR/GAPDH relative to the control group. ( D , E ) Cells were treated with various concentrations of DON (0, 0.1, 0.5, and 1 μmol/L) for 24 h, and the production of ROS and MDA levels were measured. * p < 0.05, ** p < 0.01.

Article Snippet: CYP19A1 , 1:500 , Cell Signaling, USA , AB_2630344 , 14528s , 50.

Techniques: Enzyme-linked Immunosorbent Assay, Protein Concentration, Control, Western Blot

Journal: Drug Design, Development and Therapy

Article Title: Semaglutide Alleviates Ovarian Oxidative Stress and Autophagy via the PI3K/AKT/mTOR Pathway in Mice with Polycystic Ovary Syndrome

doi: 10.2147/DDDT.S522730

Figure Lengend Snippet: (A ) Immunofluorescence images; ( B ) CYP17A1 expression area; ( C ) StAR expression area; ( D ) CYP19A1 (Immunohistochemistry); ( E ) CYP19A1 expression area. Data are presented as mean±SD. Vs NC, ## P <0.01; Vs PCOS, * P <0.05, ** P <0.01; n=6 per group.

Article Snippet: The antibodies used in the study were CYP19A1 antibodies (No. ba3704, BOSTER, China; dilution 1:50;), Beclin-1 antibodies (dilution 1:100; No.#3495, CST, USA), p62 antibodies (dilution 1:500; No. #23214, CST, USA), LC3B antibodies (dilution 1:200; No.#83506, CST, USA) was increased for half an hour and then observed.

Techniques: Immunofluorescence, Expressing, Immunohistochemistry